In-Cell Western™ 检测试剂盒和试剂,In-Cell Western™ Assay Kits and Reagents

Perform Fast, Cost-Effective Cell-Based Western Assays

The In-Cell Western Assay is an immunocytochemical assay that uses fluorescence to detect and quantify proteins in cells that have been cultured in microplates. Detecting proteins in their cellular context increases quantification precision.

This process allows for higher throughput compared to Western blotting and eliminates typical Western blotting steps such as cell lysate preparation, electrophoresis, and membrane transfer. Cost per well is reduced to a fraction of the cost of typical screening methods with CellTag™ Stain In-Cell Western Kits.

CellTag Stains are a fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells and provides linear fluorescent signal across a wide range of cell types and cell numbers. CellTag Stains are applied to the cells during incubation with IRDye^® Secondary Antibodies and enable accurate measurement of target protein levels with much higher throughput than Western blotting.

In-Cell Western 检测是一种免疫细胞化学检测，它使用荧光检测和量化微孔板中培养的细胞中的蛋白质。在细胞环境中检测蛋白质可提高定量精度。

与蛋白质印迹法相比，该过程允许更高的通量，并消除了典型的蛋白质印迹法步骤，例如细胞裂解物制备、电泳和膜转移。使用 CellTag™ Stain In-Cell Western Kits 将每孔成本降低到典型筛选方法成本的一小部分。

CellTag 染色剂是一种荧光非特异性细胞染色剂，可为 In-Cell Western 应用提供准确的细胞数标准化。该染料在透化细胞的细胞核和细胞质中积累，并在各种细胞类型和细胞数量中提供线性荧光信号。在与 IRDye® 二抗孵育期间将 CellTag Stains 应用于细胞，并能够以比蛋白质印迹高得多的通量准确测量靶蛋白水平。