PBS Blocking Buffer and PVDF Kits,PBS 封闭缓冲液和 PVDF 试剂盒
TBS Blocking Buffer and PVDF Kits,TBS 封闭缓冲液和 PVDF 试剂盒
Odyssey® Nitrocellulose Membranes,Odyssey® 硝酸纤维素膜
4X Protein Sample Loading Buffer and PVDF Membrane Kit,4X 蛋白质上样缓冲液和 PVDF 膜试剂盒
A low-background membrane is essential for infrared Western blot success. Background can be attributed to membrane autofluorescence or to detection of antibody non-specifically binding to the membrane.
低背景膜对于红外蛋白质印迹的成功至关重要。 背景可归因于膜自发荧光或检测到与膜非特异性结合的抗体。
Rely on Quality and Performance with LI-COR Western Blot Membranes
LI-COR has evaluated many different membranes for Western blotting; examples of membrane performance can be seen in the figure below. There is typically more variability in PVDF membrane performance than for nitrocellulose membranes.
LI-COR offers high-quality, prescreened Immobilon®-FL PVDF, without lot-to-lot variability, packaged in affordable kits with the Intercept™ Blocking Buffer (PBS or TBS), or with 4X Protein Sample Loading Buffer. LI-COR also offers nitrocellulose membranes in both sheets and rolls.
LI-COR 已经评估了许多不同的蛋白质印迹膜。 膜性能示例如下图所示。 PVDF 膜的性能通常比硝酸纤维素膜具有更大的可变性。
LI-COR 提供高质量、预筛选的 Immobilon®-FL PVDF，无批次间差异，采用经济实惠的试剂盒包装，带有 Intercept™ 封闭缓冲液（PBS 或 TBS）或 4X 蛋白质上样缓冲液。 LI-COR 还提供片状和卷状硝酸纤维素膜。
Membranes and Membrane Kit Components
| Product | P/N | What’s Included |
|---|---|---|
| Odyssey Nitrocellulose Membranes | 926-31090 | 0.22 µm; ten 7 cm × 8.5 cm sheets |
| Odyssey Nitrocellulose Membranes | 926-31092 | 0.22 µm; 30 cm × 3 m roll |
| PBS Blocking Buffer (500 mL) and PVDF Kit | 926-32098 | 0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (PBS) Blocking Buffer |
| TBS Blocking Buffer (500 mL) and PVDF Kit | 926-32099 | 0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (TBS) Blocking Buffer |
| 4X Protein Sample Loading Buffer and PVDF Membrane Kit | 926-31097 | 0.45 µm; 26.5 cm × 3.75 m roll + 15 mL 4X Protein Sample Loading Buffer |
| PBS Blocking Buffer (10 x 500 mL) and PVDF Kit | 829-32080 | 0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (PBS) Blocking Buffer |
| TBS Blocking Buffer (10 x 500 mL) and PVDF Kit | 926-32100 | 0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (TBS) Blocking Buffer |
Western Blot Membrane Selection Can Affect Your Results
